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The CU-Boulder 2013 team worked on a low-cost restriction enzyme expression and purification system optimized for minimal equipment and reagents use to enable simple, on-location production of BBF10-compatible REs while reducing waste flows. This led the team to demonstrate several clever shortcuts for routine molecular biology techniques, such as a protein purification protocol using an agarose gel and enzyme purification with elastin fusion proteins. Overall, their project demonstrated affordable alternatives to more costly techniques that are often taken for granted in traditional settings.