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7th August 2015
Project Title and Abstract were uploaded. Stab culture for FtsZ gene was obtained from iGem and inoculated onto Cam+ LB plates.
8th August 2015
Buffers and chemicals were prepared. Growth was observed on yesterday’s plates and further sub-culturing was done.
9th August 2015
Sequences were finalised for G-Blocks and culture plates with P. aeuriginosa [model organism] and Lemo21 [derivative of E.coli BL21]
10th August 2015
FtsZ culture were plated. ( LB Agar). Isolation, followed by Elution of Lambda DNA was carried out. Later, isolation of plasmid DNA was started. Molecular biology protocols were derived.
11th August 2015
AGE was repeated since no plasmid dna was viewed. Multiple times sub-culturing of FtsZ gene and P. aeuriginosa strains were done for cross-checking purposes. Permission letter was sent to schools and colleges for carrying out Human Practices.
12th August 2015
Each team member went to different colleges and schools for confirming dates for a presentation. Glasswares list was sorted out. Buffers and reagents were prepared.
13th August 2015
Presentation was done to our Co-ordinator till the work completed. Growth curve for both the plated samples was done but results were not obtained due to shaker incubator dis-function.
14th August 2015
CCMB buffer was prepared. Questionaries were prepared for providing it to schools and colleges (part of Human Practice).
15th August 2015
Genetic Engineering Lab was made ready. BL21 strain and FtsZ gene were inoculated on 100ml SOB broth and 50ml LB broth respectively.
16th August 2015
Wiki page framework was prepared and logo design was done on paper.
17th August 2015
Plasmid isolation was again done for confirmation. Fresh 2x100ml SOB broth was prepared.
18th August 2015
Freshly prepared SOB broth was inoculated with 1 BL21 colony from the respective plate. Logo was further reviewed. Collaboration deadlines were made.
19th August 2015
Plasmid isolation for pET24 was done. Presentation was done at Arulmigu Meenakshi Amman Engieering College. Funding list of companies was done. Growth curve for P. aeruginosa was completed. BL21 gram staining was done.
20th August 2015
Seed stock for BL21 was prepared. App design was done on paper. Primers required for the project was designed and ordered. Further pET24 was inoculated.
21st August 2015
Growth curve for BL21 strain was completed. pET24a transformation was done. Presentation was done at Prathyusha Institute of Technology.
22nd August 2015
Dr. Kannan of Saveetha Medical College was approached. Interviewed him and he gave information about antibiotic resistance. A video was recorded for the same.
23rd August 2015
Collaboration with Team Uppsala was confirmed. Basic Wiki page update was done.
24th August 2015
Poster design was given. Kan+ transformation plates were prepared. Final safety forms were prepared. pSB1C3 gene was transformed. P. aeruginosa staining was done. Certified by IEC.