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  • Team:Aachen/Notebook/Documentation/Glycogen Knockout Generation
    * colony PCR using #A9W9# and #XE3D# to verify plasmid curing ! construct & clone !! cryo ID !! genomic DNA ID !! PCR product !! purified PCR product !! sequencing result
    34 KB (4,858 words) - 20:27, 18 September 2015
  • Team:Aachen/Notebook/Documentation/Glycogen Targeting Plasmids
    ** to be amplified by PCR from pTargetF ** to be assembled from genomic PCR of left and right homology arm and primers with overhangs (IGEM AACHEN)
    16 KB (2,268 words) - 17:50, 18 September 2015
  • Team:NEFU China/Notebook
    ...bsp;Kit&nbsp;(see&nbsp;protocol).&nbsp;The&nbsp;concentration&nbsp;of&nbsp;genomic&nbsp;DNA&nbsp;was&nbsp;measured&nbsp;with&nbsp;a&nbsp;NanoDrop&nbsp;spectro <p><span style="font-family:arial,helvetica,sans-serif">1.PCR</span></p>
    87 KB (13,477 words) - 18:31, 18 September 2015
  • Team:ZJU-China/Project/Protocol
    <h5 class="hh4-left" style="padding-top:30px" >Streptomyces genomic DNA extraction</h5> <h5 class="hh4-left" style="padding-top:30px">PCR</h5>
    37 KB (4,772 words) - 14:38, 17 September 2015
  • Team:Peking/Design/Isothermal
    ...nealing and extension, simulating <i>in vivo</i> DNA replication progress. PCR has radically altered molecular biology; via this technique, a trace amount ...ously. All these methods need a thermal cycler; Nested PCR and Multiplexed PCR need more than two pairs of primers, which may lead to the generation of pr
    39 KB (4,417 words) - 14:54, 17 November 2015
  • Team:Aachen/Notebook/Protocols
    # pipet a Phusion polymerase PCR of 50&nbsp;µl total volume # PCR
    29 KB (4,409 words) - 03:37, 19 September 2015
  • Team:Michigan/Experiments
    <h3>QIAquick PCR Purification Kit Protocol using a microcentrifuge</h3><br> ...otocol is designed to purify single- or double-stranded DNA fragments from PCR and other enzymatic reactions. Fragments ranging from 100 bp to 10 kb are p
    18 KB (2,790 words) - 07:10, 20 November 2015
  • Team:NEFU China/Satety
    ...ans-serif">According to the World Health Organization Laboratory Biosafety Manual, the rank of our lab is classified as BSL1. Each member was informed of the ...of Salmonella Typhimurium str. LT2 and amplified these essential genes by PCR. We used Escherichia coli MC1601 for molecular cloning. Besides, we culture
    5 KB (717 words) - 18:03, 17 September 2015
  • Team:NEFU China/Safety
    ...ans-serif">According to the World Health Organization Laboratory Biosafety Manual, the rank of our lab is classified as BSL1. Each member was informed of the ...almonella Typhimurium</em> str. LT2 and amplified these essential genes by PCR. We used <em>Escherichia coli</em> MC1601 for molecular cloning. Besides, w
    6 KB (869 words) - 23:33, 18 September 2015
  • Team:NEFU China/Safty
    ...ans-serif">According to the World Health Organization Laboratory Biosafety Manual, the rank of our lab is classified as BSL1. Each member was informed of the ...of Salmonella Typhimurium str. LT2 and amplified these essential genes by PCR. We used Escherichia coli MC1601 for molecular cloning. Besides, we culture
    5 KB (808 words) - 22:56, 18 September 2015
  • Team:York/ProjectBreakdown
    ...is has enabled the production of phylogenetic analysis and mostly-complete genomic sequence assemblies. We decided to tap into the PAOs potential and systemat ...re carrying out growth assays with these particular mutants we used colony PCR to verify the knockouts. This showed that the mutations were correct in the
    41 KB (6,201 words) - 03:40, 19 September 2015