Team:Aalto-Helsinki/InterLabBook

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• Week 1

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Interlab Measurement Study Lab Book

Before reading our Interlab lab book, take a look into this excel file to find the nomenclature we used for our devices.

Week 1

Worked With	WorkFlow						June 22nd - June 26th
Preparations	Transform AHD1-AHD4 into TOP10	o/n culture	MiniPrep
	Use iGEM's linearized backbones
Assembly	3A assembly for D1-D3	Stored in -20°C

Click here to read the full lab notes of week 1.

Week 2

Worked With	WorkFlow						June 29th - July 3rd
Preparations	Transform AHD6 & AHD7 into TOP10	o/n culture	MiniPrep
Assembly	Transform D1-D3 into TOP10	D2 expressing GFP	Refresh positive colonies	No GFP
		No colonies for D1 & D3	Re-assemble D1-D3	Transform into TOP10	D2 successful	o/n culture	MiniPrep
					No colonies for D1 & D3

Click here to read the full lab notes of week 2.

Weeks 3-5

Worked With	WorkFlow						July 6th - July 24th
Checking D2	Transform D2 into TOP10	GFP expressed
Assembly	Re-restrict AHD1 & AHD3-AHD5	Stored in -20°C

Click here to read the full lab notes of weeks 3-5.

Week 6

Worked With	WorkFlow						July 27th - July 31st
Assembly	Ligations for D1 & D3	Transform into TOP10
Checking	Check AHD1 & AHD3-AHD5 restrictions on gel	AHD4 & AHD5 OK	Too little restriction mix for new ligation	Re-restrict	Stored in -20°C
Assembly	Ligations for D1 & D3	Transform into TOP10
		AHD1 & AHD3 unclear	Re-restrict	Stored in -20°C

Click here to read the full lab notes of week 6.

Week 7

Worked With	WorkFlow						August 3rd - August 7th
Assembly	Ligations for D1 & D3	Transform into TOP10	D1 expressing GFP	o/n culture	MiniPrep
		No GFP expression in D3	Re-restrict AHD3-AHD5	Ligate & transform into TOP10	No GFP expression
Checking	Mistake in AHD4 restriction protocol	Check AHD4 restriction on gel	Restriction OK

Click here to read the full lab notes of week 7.

Weeks 8-10

Worked With	WorkFlow						August 10th - August 28th
Measurement	Transform D1 & D2 into TOP10	o/n cultures	Measure GFP
Checking	Restrict & check D1 & D2 on gel

Click here to read the full lab notes of weeks 8-10.

Due to the limited time we had to use for our project, we were only able to construct two devices and measure the GFP from these. We were unable to build the device with J23106 promoter.

Our results can be found on our Interlab Study main page.