Team:Bielefeld-CeBiTec/Primers
Primers
Small in size, but great in performance!
| Name | Sequence (5´ → 3´) | |
|---|---|---|
| Standard primers | ||
| mRFP1_rev | GGACGACCTTCACCTTCACCTTCACC | |
| SB-prep-2Ea | ATGAATTCCAGAAATCATCCTTAGCG | |
| SB-prep-3P-1 | GCCGCTGCAGTCCGGCAAAAAA | |
| VF | CCACCTGACGTCTAAGAA | |
| VR | GTATTACCGCCTTTGAGT | |
| Arsenic biosensor | ||
| Ars_1_rev | TATGACTTAACGAATGTGTAAGTGCAGAGGCGGATAAAACACATCTGAAAATTC | |
| Ars_2_fwd | CCTCTGCACTTACACATTCGTTAAGTCATAGGTACTAGTAGCGGCC | |
| Ars_3_fwd | CATTCGTTAAGTCATATATGTTTTTGACTTGGTACTAGTAGCGGCC | |
| Ars_3_rev | TCAAAAACATATATGACTTAACGAATGTGTCGGATAAAACACATCTGAAAATTC | |
| arsRhis_arsR_fwd | TATAGGGAAAGAGGAGAAATACTAGAGATGTCATTTCTGTTACCCATC | |
| arsRhis_arsR2_r | CTAGTATTAGTGGTGATGGTGATGATGACTGCAAATGTTCTTACTG | |
| arsRhis_Trp_fwd | CATCATCACCATCACCAC | |
| arsRhis_Trp_rev | CTCTAGTATTTCTCCTCTTTCC | |
| UTR_arsO_rev | CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTAAGTCAAAAACATATATGACTTAACG | |
| UTR_arsR_fwd | GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGTCATTTCTGTTACCCATCC | |
| UTR_arsR_rev | CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTCCCTATAGTGAGTCGTATTACTC | |
| UTR_arsR_fwd | GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGTCATTTCTGTTACCCATCC | |
| UTR_arsR_rev | CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTCCCTATAGTGAGTCGTATTACTC | |
| CFPS | ||
| Mut_gap_UTR_fwd | AGAATAATCTATGCGTAAAGGCGAAGAG | |
| pBS1C3_RraA_pre | ACAAAGCTCGGAAGTATCGTATTTCATCTCTAGAAGCGGCCGCGAATTCC | |
| pBS1C3_RraA_suf | TCAGAAGATCCGCTGGATATTGAATGATACTAGTAGCGGCCGCTGCAG | |
| pBS1C3_RraB_pre | TTCTTCCAGTTGTTCCGGGTTTGCCATCTCTAGAAGCGGCCGCGAATTCC | |
| pBS1C3_RraB_suf | GAAGACGATGACGGAGTTCGCCACTAATACTAGTAGCGGCCGCTGCAG | |
| pZ9_pT7_rne_fwd | GTCCGCAACCTGTTGAGTAACTGCAGGAAGGGGATCCG | |
| pZ9_pT7_rne_rev | TAGTATTTCTCCTCTTTCTCTAGTATCTCCCTATAGTGAGTCGTATTAC | |
| rne_qPCR_fwd1 | GTGCTCGGCATAAGCCGAAG | |
| rne_qPCR_fwd2 | GCATAAGCCGAAGATATCG | |
| rne_qPCR_fwd3 | CCGAAGATATCGGTAGAGTT | |
| rne_qPCR_fwd4 | CTGCGCTACGGTAGCGAAAC | |
| rne_qPCR_rev2 | TTTGCGCCTCGTTATCATCC | |
| rne_to_pZ9_fwd | GATACTAGAGAAAGAGGAGAAATACTAGATGAAAAGAATGTTAATCAACGCAACTC | |
| rne_to_pZ9_rev | CCTGCAGTTACTCAACAGGTTGCGGACGCGCAG | |
| Rra_purif_fwd | TCAGAAGATCCGCTGGATATTGAATGATACTAGAGCCAGGCATCAAA | |
| RraA_fwd | ATGAAATACGATACTTCCGAGCTTTG | |
| RraA_purif_rev | ACAAAGCTCGGAAGTATCGTATTTCATGGCGCCCTGAAAATACAG | |
| RraA_rev | TCATTCAATATCCAGCGGATCTTC | |
| RraB_fwd | ATGGCAAACCCGGAACAACTG | |
| RraB_purif_fwd | GAAGACGATGACGGAGTTCGCCACTAATACTAGAGCCAGGCATCAAA | |
| RraB_purif_rev | TTCTTCCAGTTGTTCCGGGTTTGCCATGGCGCCCTGAAAATACAG | |
| RraB_rev | TTAGTGGCGAACTCCGTCATC | |
| SYFP_only_fwd | ATGGTTAGCAAGGGCGAAGA | |
| SYFP_only_rev | TTATTATTTATACAGCTCATCCATACC | |
| UTR_noT7_rev | CCTTCTTTTTTTTTTTTAAAGTTAAAGTTAAACAAAATTATTCTCTAGAAGCGGCCGCGA | |
| UTR_fwd | GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGCGTAAAGGCGAAGAGCTGTTCAC | |
| UTR_rev | CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTTCTCCCTATAGTGAGTCGTATTACTCTAGAAG | |
| UTR_RFP_fwd | GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTATGGCTTCCTCCGAAGACGTTATC | |
| UTR_SYFP_fwd | GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTATGGTTAGCAAGGGCGAAGAAC | |
| Date rape drugs | ||
| BlcR_fwd | ATGGGCCAAAGGGGCCAAGT | |
| BlcR_rev | CTAGTCTTTCTGCGATCGGTACCC | |
| pSB1C3_BlcR_fwd | CTTGGGTACCGATCGCAGAAAGACTAGTACTAGTAGCGGCCGCTGCAG | |
| psB_BlcRfwd_new | CTTGGGTACCGATCGCAGAAAGACTAATACTAGTAGCGGCCGCTGCAG | |
| pSB1C3_BlcR_rev | TTGACACACTTGGCCCCTTTGGCCCATCTAGTATTTCTCCTCTTTCTCTAGTAGCTAGCACAATAC | |
| BlcC_rev | TTACGCGTAAAATTCGGGAGCCTTC | |
| BlcC_rev_new | TTAGTCTTTCTGCGATCGGTACCC | |
| bclR-rv | GTCTTTCTGCGATCGGTACCC | |
| blcR-CBD-fw | AAGCTTGGGTACCGATCGCAGAAAGACCCCGGCGCCAATCCGCCCGGT | |
| pro_Blc_fwd | ATGCACCTTCCTTGACACACT | |
| pro_Blc_rev | CATCGACCTCTCCCGCATTAG | |
| K516030proB_rev | GGGCCAAGTGTGTCAAGGAAGGTGCATCTCTAGAAGCGGCCGCGAATTCC | |
| K516030proB_fwd | TGAACTCTAATGCGGGAGAGGTCGATGGCTTCCTCCGAAGACGTTAT | |
| BlcB_mut_fwd | CCTTTCGAGTTCCGCACCGTTCCCT | |
| BlcB_mut_rev | AGGGAACGGTGCGGAACTCGAAAGG | |
| BlcR-L-sfGFP-rv | TCGCCTTTACGCATGCCGGTGTCTTTCTGCGATCGGTACCC | |
| BlcR-T7-RBS-fw | TAGAGAAAGAGGAGAAATACTAGATGGGCCAAAG | |
| K60_BlcPshort_r | GAGTGAACTATGGCTCTAGTAGCTAGCACAATACC | |
| K60_BlcPshort_f | GCTAGCTACTAGAGCCATAGTTCACTCTAATGATTC | |
| bclR-rv | GTCTTTCTGCGATCGGTACCC | |
| pSB1C3blcOnewfw | AAGAAGGCTCCCGAATTTTACGCGTAATACTAGTAGCGGCCGCTGCAG | |
| Pblc_UTR_fwd | TCAAGTTCAATTCGTTGCACTCTAATGAATAATTTTGTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATC | |
| Pblc_UTR_rev | ATTGAACTTGAATCATTAGAGTGAACTCCCTATAGTGAGTCGTATTACTCTAG | |
| BlcR-His_fwd | CGTACTACCATCACCATCACCATCACGGCGCCATGGGCCAAAGGGGCC | |
| BlcR-His_rev | CCGTGATGGTGATGGTGATGGTAGTACGACATCTAGTATTTCTCCTCTTTCTCTAGTA | |
| gadR-qRT-fw | TCAATCCCCTGTAACGG | |
| gadR-qRT-rev | CCAGGCGCACCGAACAAGAC | |
| gadT-qRT-fw | ATGAGTCAAACAACAGCAAGCATC | |
| gadT-qRT-rev | CTCAACCACCTTCGGATG | |
| oligo_BlcR_51_rev | CCCGCATTAGAGTTCAACTAATTGAACTTGAATCATTAGAGTGAACTA TGG | |
| oligo_BlcR_51_fwd _biot | Biotin_CCATAGTTCACTCTAATGATTCAAGTTCAATTAGTTGAACTCT AATGCGGG | |
| oligo_BlcR_51_rev_Cy3 | Cy3_CCCGCATTAGAGTTCAACTAATTGAACTTGAATCATTAGAGTGAACTA TGG_Cy3 | |
| oligo_BlcR_51_fwd _Cy3 | Cy3_CCATAGTTCACTCTAATGATTCAAGTTCAATTAGTTGAACTCT AATGCGGG_Cy3 | |
| gBlocks | ||
| GABA_B_pSB1C3_f | ATAAGAAAATACCGACAGGGGGATATTCACTACTAGTAGCGGCCGCTGC | |
| GABA_B_pSB1C3_r | CATAAAAAAATCCCAGTCACAGGTGATTGACTCTAGAAGCGGCCGCGA | |
| GABA_R_pSB1C3_f | TAGTTTTCAACCCTTTCAGCGGAGGAGCCATCGTACTAGTAGCGGCCGCTGC | |
| GABA_R_pSB1C3_r | CTGTGGATTAACACACCACCTACTTTTTAACTCTAGAAGCGGCCGCGA | |
| Trp_sy_pSB1C3_f | AAAGAAGTTCATCATCACCATCACCACTAATACTAGTAGCGGCCGCTGC | |
| Trp_sy_pSB1C3_r | TCTCCTCTTTCCCTATAGTGAGTCGTATTACTCTAGAAGCGGCCGCGA | |
| Heavy metals | ||
| cueR_rev | TCACCCTGCCCGATGATGAC | |
| cueR_fwd | AGTCCTAGGTATTGTGCTAGCTACTAGAGAAAGAGGAGAAAATGAACATCAGCGATGTAGC | |
| rnR_PstI_fwd | CGAATGCGCAGTTTTAC | |
| rnR_PstI_rev | GTAAAACTGCGGCGCATTCG | |
| pSB1C3_copAP_fwd | TTGACCTTCCCCTTGCTGGAAGGTTTAACCTTTATCACAATTAAAGAGGAGAAATACTAG | |
| pSB1C3_copAP_rev | TAAACCTTCCAGCAAGGGGAAGGTCAAAGAAATTAATAAACTCTAGAAGCGGCCGCGAAT | |
| pSB1C3_copA_rev | GGTCAAGAAATTAATAAACCAGGCGGGTCTCTAGAAGCGGCCGCGAAT | |
| Chrom2_pSB1C3_fwd | AGCTTTGAAAAAGGGACCCTCACCGTATGATACTAGTAGCGGCCGCTGC | |
| Chrom2_pSB1C3_rev | AATAAAAGCAAATGGCAATAGGAATAAGCAATCCTCTAGAAGCGGCCGCGA | |
| pSB1C3_cueR_pre | GGCTGCTGTCATCATCGGGCAGGGTGACTCTAGAAGCGGCCGCGAAT | |
| pSB1C3_cueR_suf | CTAGTAGCTAGCACAATACCTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA | |
| pSB1C3_cueR_rev | GGTTGTTGTCATCATCGCGCAGGGTAACTCTAGAAGCGGCCGCGAAT | |
| pSB1C3_rcnA_fwd | TAGATTAATAGTGCTATGATTTTTTATTGTTAACCAGGTGTTGCCATGTCTCATCTCTAGAAGCGGCCGCGAAT | |
| pSB1C3_rcnA_rev | TGAAAAATCATAGCACTATTAATCTAGGGGGGTAGTATCAGGTACTGGGGGGGAGTAATTAAAGAGGAGAAATACTA | |
| pSB1C3_rcnR_rev | AGTCCTAGGTATTGTGCTAGCTACTAGAGAAAGAGGAGAAAATGTCTCATACAATCCGTGATA | |
| pSB1C3_rcnR_fwd | CGCGGCCGCTTCTAGAGTTACTTGATATATGAATCCAGC | |
| pSB1C3_rcnR_f | CTAGTAGCTAGCACAATACCTAGGACTGAGCTAGCTGTAAATACTAGTAGCGGCCGCTGCAGTCC | |
| pSB1C3_rcnR_rev | GAAAGTCCTTGATTCGTACATCAAATGACTCTAGAAGCGGCCGCGAAT | |
| pSB1C3_merR_suf | CTAGCACAATACCTAGGACTGAGCTAGCTGTCAATAGTAGTAGCGGCCGCTGCAG | |
| pSB1C3_merR_pre | GGCCTGGCAAGGTCAGCTAGTCCTTAGCTCTAGAAGCGGCCGCGAAT | |
| rcnA_UTR_fwd | CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTTTTCTCCTCTTTTACTCCCCCC | |
| UTR_mod_fwd | GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTCTGCGTAAAGGCGAAGAGCTGTTCAC | |
| MerR_fwd | TAGCTCAGTCCTAGGTATTGTGCTAGCAAAGAGGAGAAATACTAGATGG | |
| merR_rev | CTAAGGCATAGCTGATCTTG | |
| copAP_UTR_rev | CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTCTAGTACTTTAAGACAGTTTTG | |
| CrProm_UTR_rev | CTTTTTTTTTTTTAAAGTTAAACAAAATTATTGTCGTAGATGTTACTACAATAATGAG | |
| pbrA_UTR_rev | CTCCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTCTAGTATTTCTCCTCTTTTTCTCGCGTTGCC | |
| pSB1C3_merT_rev | GGATAGCGTAACCTTACTTCCGTACTCATGTACGGAGTCAAGCGATATGGAACTCTAGAAGCGGCCGCGAATTC | |
| merT_omRFP_fwd | CTCCGTACATGAGTACGGAAGTAAGGTTACGCTATCCAATCCTACTAGATTAAAGAGGAGAAATAC | |
| 1C3_pre_merT_rev | CTTACTTCCGTACTCATGTACGGAGTCAAGCGATATGGAACTCTAGAAGCGGCCGCGAATTC | |
| UTR_merT_fwd | GAGTACGGAAGTAAGGTTACGCTATCCAATCCTACGACAATAATTTTGTTTAACTTTAAAAAAAAAAAAG | |
| pSB1C3_kPrm_fwd | CTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA | |
| pSB1C3_kPrm_r | GCACCACCGCGCACCCGTCCGATTAACTCTAGAAGCGGCCGCGAAT | |
| pSB1C3-pbrR-fwd | CCACCGCGCACCCGTCCGATTAACTCTAGAAGGGCCGCGAAT | |
| pSB1C3-pbrR-f | CAATACCTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA | |
| pSB1C3-pbrA_rev | CTATAGAGTCAAGACATCTCCCACTCTAGAAGCGGCCGCGAAT | |
| pSB1C3_RFP_rev | CCGCCACTCAACGGGTGCCTGATAATACTAGTAGCGGCCGCTGCA | |
| pSB1C3_rcnA_rev | CTTGTTAACCAGGTGTTGCCATGTCTCATCTCTAGAAGCGGCCGCGAAT | |
| pSB1C3_merR_rev | CGGTCTTGCCCGGAGCGCAATGCCGTGACTCTAGAAGCGGCCGCGAAT | |
| pSB1C3_chrB_rev | CTTTTGAAAAAGGCACTCTGACCGTTTGACTCTAGAAGCGGCCGCGAAT | |
| pSB1C3_chrP_rev | CAAATGGCAATAGGAATAAGCAATCCTCTAGAAGCGGCCGCGAAT | |
| PRIA | ||
| T7-RBS-arsR-fw | AGGGAGATACTAGAGAAAGAGGAGAAATACTAGATGTCATTTCTGTTACC | |
| arsR-L-sfGFP-rv | CAGCTCTTCGCCTTTACGCATGCCGGTTAACTGCAAATGTTCTTAC | |
| L-sfGFP-fw | ACCGGCATGCGTAAAGGCGAAGAGCTG | |
| T7-RBS-arsR-rv | CTAGTATTTCTCCTCTTTCTCTAGTATC | |
| lacI-sfGFP-fw | CCCGACTGGAAAGCGGGCAGACCGGCATGCGTAAAGGCGAAGAGC | |
| lacI-sfGFP-His-rv | TAATGGTGATGGTGATGGTGTTTGTACAGTTCATCCATACCATGCGTGATGC | |
| T7-RBS-lacI-rv | CTGCCCGCTTTCCAGTCGGGAAACCTGTC | |
| C3-lacI-His-fw | AACACCATCACCATCACCATTAATAA | |
| UberHis-arsR-fw | CACCATCACCATCACCATTAATAATACTAGAGCCAGGCATCAAA | |
| arsR-UberHis_rv | TTATTAATGGTGATGGTGATGGTGTTTGTACAGTTCATCCATACC | |
| BlcR-T7-RBS-fw | TAGAGAAAGAGGAGAAATACTAGATGGGCCAAAG | |
| BlcR-L-sfGFP-rv | TCGCCTTTACGCATGCCGGTGTCTTTCTGCGATCGGTACCC | |
| arsO-fw | Aminolink_ACATTCGTTAAGTCATATATGTTTTTGACTTA | |
| arsO-rv | Cy3_TGTAAGCAATTCAGTATATACAAAAACTGAAT | |
| SuffixR-fw | Aminolink_CTGCAGCGGCCGCTACTAGTA | |
| lacO-klein | ATTTGCTTTGTGAGCGGATAACAAT | |
| lacO-gross | TGTGGAATTGTGAGCGGATAACAATTACG | |
| lacI-CBD-fw | GACAGGTTTCCCGACTGGAAAGCGGGCAGCCCGGCGCCAATCCGCCCGGTACTACG | |
| lacI-CBD-Vektor-rv | GGACTGCAGCGGCCGCTACTAGTATTATTAAAGACACTGGGAGTAGTACG | |
| EndHis-Vektor-lacI-fw | TAATAATACTAGTAGCGGCCGCTGCAGTCC | |
| lacI-rv | CTGCCCGCTTTCCAGTCGGGAAACC | |
| arsR-dCBD-fw | GGGACAGTAAGAACATTTGCAGTTACCCGGCGCCAATCCGCCCGGTAC | |
| CBD-eHisTerm-rv | TATTTGATGCCTGGCTCTAGTATTATTAAAGACACTGGGAGTAGTACG | |
| eHis-dTerm-fw | TAATAATACTAGAGCCAGGCATCAAA | |
| arsR-rv | TAACTGCAAATGTTCTTACTG | |
| bclR-CBD-fw | AAGCTTGGGTACCGATCGCAGAAAGACCCCGGCGCCAATCCGCCCGGTACT | |
| CBD-eGFPTerm-rv | ATTTGATGCCTGGCTCTAGTATCATCAAAGACACTGGGAGTAGTACG | |
| eGFP-dTerm-fw | TGATGATACTAGAGCCAGGCATCAAAT | |
| bclR-rv | GTCTTTCTGCGATCGGTACCC | |
| lacIhis0418_fw | GGGCAGCACCATCACCATCACCATTAATAATACTAGTAGCGGCCGCTGC | |
| lacIhis0418_rv | TTATTAATGGTGATGGTGATGGTGCTGCCCGCTTTCCAGTCG | |
| VF-Cy3 | Cy3-CCACCTGACGTCTAAGAAAC | |
| arsO-rv_Cy3 | Cy3_TGTAAGCAATTCAGTATATACAAAAACTGAAT | |
| Split primers | ||
| Cm_fwd | CGGCATCAGCACCTTGTC | |
| Cm_rev | TATACGCAAGGCGACAAG | |
