Protocols Our Organisms of Choice is Escherichia coli and Saccharomyces cerevisiae . Listed below are the protocols we used in our lab. Cloning PCR Methods iGEM Bordeaux E.coli Competent Cells Plasmid Extraction using Alkaline Lysis Method Working with E.coli Growing Media of E.coli E.coli Curdlan Purification Working with S.cerevisiae High Efficiency Yeast Transformation Genomic DNA Preparation from Yeast Curdlan Methods Curdlan Quantification Sulfation of Curdlan by Sodium Pyrosulfate in Dimethyl Sulfoxide
Our Organisms of Choice is Escherichia coli and Saccharomyces cerevisiae . Listed below are the protocols we used in our lab.