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Notebook

Calendar

June

Monday Tuesday Wednesday Thursday Friday Saturday Sunday
01/06 02/06
  • - iGEM kit arrived!
    		•  03/06 04/06 05/06 06/06 07/06
    08/06 09/06
    - Transformation:
    - B0034
    - K1497008
    - R0010
    - K914003
    - R0080
    - C0040
    - B0015
    - R0040
    - E0840
    - E0040
    - P0440
    - I13507
         		10/06 11/06
    - Inoculum
    - B0034
    - K1497008
    - R0010
    - K914003
    - R0080
    - C0040
    - B0015
    - R0040
    - E0840
    - E0040
    - P0440
    - I13507
         		12/06
    - Miniprep and Digestion EXSP
    - B0034
    - K1497008
    - R0010
    - K914003
    - R0080
    - C0040
    - B0015
    - R0040
    - E0840
    - E0040
    - P0440
    - I13507
         		13/06


    - Agarose gel (Problems with the buffer! Way too concentrated ><)
    - Digestion EXSP again (C0040, B0015, R0040, E0840, E0040, P0440, I13507) [We didn’t have B0034 anymore, so we didn’t prepare the reaction for K1497008,R0010, K914003, R0080]

    		 14/06


    - Agarose gel(C0040, B0015, R0040, E0840, E0040, P0440, I13507)
    - Gel purification (C0040, B0015, E0040, P0440)
    - Interlab transformations (J23101,J23106,J23117,I13504)
    - Ligation Tet_term (C0040 + B0015)
    - Ligation GFP_P0440 (E0040 + P0440)

    15/06
    - Transformation Tet_term (C0040 + B0015)
    - Transformation GFP_P0440 (E0040 + P0440
    - Interlab inoculum
    - Inoculum (B0034)     		16/06
    - Inoculum (Tet_term, GFP_P0440)
    - Inoculum (Tet_term, GFP_P0440)
    - Interlab minipreps
    - Miniprep (B0034)
    - Digestion ESXP (B0034, B0034, K1497008, R0010, K914003, R0040, E0840)     		17/06
    - Miniprep (Tet_term, GFP_P0440)
    - Agarose gel (B0034, B0034, K1497008, R0010, K914003, R0040, E0840)
    - Gel purification (B0034, B0034, K1497008, R0010, K914003, R0040, E0840)
    - Ligation RBS_hokD (B0034 + K1497008)
    - Ligation Pl_RBS (R0010 + B0034)
    - Ligation Pt_E0840 (R0040 + E0840)
    - Ligation Pt_I13507 (R0040 + I13507)     		18/06
    - Transformation (RBS_hokD, Pl_RBS, Pt_E0840, Pt_I13507)     		19/06
    - No colonies! Wrong antibiotic!!!!
    - New transformation (RBS_hokD, Pl_RBS, Pt_E0840, Pt_I13507)     		20/06
    - Inoculum (Pl_RBS, Pt_E0840, Pt_I13507)
    - RBS_hokD didn’t grow     		21/06
    - Miniprep (Pl_RBS, Pt_E0840, Pt_I13507)
    - Digestion NotI (Tet_term, GFP_P0440, Pl_RBS, Pt_E0840, Pt_I13507)
    22/06
    - Confirmation, agarose gel (Tet_term, GFP_P0440, Pl_RBS, Pt_E0840, Pt_I13507)
    - Confirmed (Tet_term, Pl_RBS, Pt_E0480)
    - Ligation (GFP_P0040, Pt_I13507, RBS_hokD)     		23/06
    - Transformation (GFP_P0040, Pt_I13507, RBS_hokD)     		24/06
    - Inoculum [GFP_P0040 (3 colonies), RBS_hokD (3 colonies), I13507, R0080, K914003]     		25/06
    - Miniprep [GFP_P0040 (3 colonies), RBS_hokD (3 colonies), I13507, R0080, K914003]     		26/06 27/06
    - EXSP digestion (K91403)
    - Confirmation digestion (RBS_hokD)     		28/06
    - Agarose gel (K91403)
    - Agarose gel (RBS_hokD) – positive result \o/
    - Gel purification (K91403)
    - Ligation Pr_RBS (K91403 + B0034) 22ºC for 4h, 16ºC for 10h, 4º overnight
    - EXSP digestion RBS_hokD
    - Interlab digestion (from minipreps of June 15th)
    - Promoters J23101,J23106 and J23117 - SP enzymes
    - GFP part I13504(RBS B0034+GFP E0040+double Term B0010 and B0012 ) - XP enzymes
    29/06
    - Agarose gel RBS_hokD
    - Ligation (Pr_RBS, Pa_RBS)     		30/06
    - Inoculum RBS_hokD
    - EXSP digestion RBS_hokD

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