Characterizing the pBad promoter using RFP promoter Notes before starting This is a method to determine relative fluorescence by eye We recommend arabinose concentrations of 0% to 0.2%, increasing in 0.04% increments Day 1 Set up overnights Add appropriate antibiotics Add arabinose in increasing amounts Let cells grow for at least 12 hours Day 2 Spin cells down for 3 minutes at 8,000x g Decant Order tubes in order of arabinose concentration Compare the fluorescence by eye Take photo to document
