Team:Cornell/protocols

Protocols

DNA Purification

• EZNA Miniprep
• Qiagen Miniprep
• Qiagen Gel Extraction
• Qiagen Column Purification

Plasmid Insertion

• Preparing Electrocompetent E.coli
• E.coli Heat Shock
• Preparing Chemically Competent E.coli
• E.coli Electroporation

PCR

• PCR Phusion

Growth Tests

• Zone of Inhibition Assays
• Lysate Assay

DNA Assessment

• DNA Gel Electrophoresis
• Preparation of DNA for Sequencing

Plasmid Editing

• Double Digest
• Vector Dephosphorylation
• T4 Ligase Ligation E.coli
• Gibson Assembly E.coli

Protein Identification and Expression

• Western Blot
• SDS-PAGE
• SDS-PAGE (2)

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