Team:ETH Zurich/Achievements

"What I cannot create I do not understand."
- Richard Feynmann


Awards and Medals

At the Giant Jamboree in Boston, MA, end of September 2105 our project MicroBeacon achieved Gold Medal status and was awarded the prize for the Best Model for our extensive modeling efforts as well as the prize for the Best Part Collection for our synthetic promoter library. Furthermore, we were nominated for the Best Health & Medicine Project, as well as the Best Wiki, Best Poster, and Best Basic Part.

We are proud to present the large variety of our further accomplishments:

General Achievements

  • Our engineered E.coli can detect CTC based on their elevated lactate output.

  • We designed a novel system for detection of circulating tumor cells in blood samples using genetically modified bacteria.

  • We designed a genetic circuit that integrates two different cancer specific signals (lactate and AHL) in an AND gate.

  • We designed and validated a tight AND gate with a clear binary behavior.

Experimental Achievements

Modeling Achievements

Human Practices and Collaboration Achievements

Medal Criteria

We registered for iGEM, had a great summer so far, and now we are looking forward to attending the Giant Jamboree!

Going for it!

We completed and submitted the Judging Form.

We created a description of our project in time.

We documented all the parts taken from the Registry of Standard Biological Parts, of which two (BBa_C0160 and BBa_K822000) were redesigned and newly characterized.

We are going to present a poster and give a talk at the Giant Jamboree.

Going for it!

We created this website for you to learn about every aspect of our iGEM project.

We documented and submitted two new basic parts to the iGEM parts registry and created a part collection with 13 parts.

These 13 new parts for our parts collection we also submitted to the iGEM Parts Registry.

We characterized two newly designed hybrid promoters and were able to show that one of our combined promoters, Plac-lldR (K1847010), reacts in a clear AND gate fashion to a combination of lactate and IPTG. To our knowledge, combining these two elements has never been attempted before.

Our Human Practices Efforts:

We visited two different elementary schools, thaught the children about what DNA is, performed experiments with them and published an article about it in the local newspaper.

We informed the ETH-student magazine polykum about iGEM and gave an interview.

We contributed to the Newsletters from Amoys team, met with the Darmstadt team, conducted a survey together with the EPFL and provided Colombias team with protocols and troubleshooting advice for their transformations.

More Human Practices Efforts:

We interviewed many different experts from various fields: medical doctors, an expert from the ethics commission of the ETH Zurich, the founder of a startup biotech company as well as an expert in patent law and integrated their advice and ideas into our project design.

We collaborated with the team from Stockholm by testing their constructs.

We Improved and characterized variants of the E. coli lldPRD-operon promoter based on the natural version (BBa_K822000), on which there is only a limited amount of information available in the Parts Registry and in the literature. The characterization of a synthetic promoter library yielded promoter variants that far outperform the wild type LldPRD promoter.

We would like to thank our sponsors