Team:Groningen/Notebook/Backbone miniprep t25
Blue Bio Energy
Miniprep RFP backbone (t25)
Miniprep was performed to obtain the plasmid DNA (RFP backbone) from E. coli.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The RFP backbone was miniprepped.
Plasmid DNA purification
00:00, 01 May 2015 - 00:00, 01 May 2015
The E. coli culture was miniprepped (Thermo Scientific #K0502, lot: 00272723) and stored as: ‘RFP backbone’ in -20°C (50µL, 90ng/µL DNA).