Team:Groningen/Notebook/GFP Transformation zp61
Blue Bio Energy
GFP Transformation (zp61)
Transformation was done to introduce the ligated plasmid DNA into E. coli.
To create a salt inducible promotor.
Transformation failed.
Heat shock transformation
00:00, 24 Augsut 2015 - 00:00, 24 August 2015
Competent E. coli were transformed with 10 µL ligation product and plated on LB plate with chloramphenicol (pSB1C3). Transformation failed.