Team:Groningen/Notebook/Ligation fr3
Blue Bio Energy
Restriction ferritin (fr3)
Restriction of ferritin
Development ferritin
Restricted product
Restriction
16:00, 31 August 2015 - 18:30, 10 August 2015
Digest ferritin in order to ligate them. Samples were digested for 2 hours at 37 °C.
Component
Amount
Ferritin
10 µL
10x buffer (2.1)
2 µL
PstI
1 µL
EcoRI
1 µL
\( \mathrm{H_2O}\)
6 µL
Components for digestion.