Team:Groningen/Notebook/Ligation fr5
Blue Bio Energy
Ligation ferritin in pSB1C3(fr5)
Ligation of ferritin in pSB1C3
Development ferritin
Ligated product
Ligation
20:15, 31 August 2015 - 22:30, 10 August 2015
Ferritin dig was ligated into pSB1C3 dig. Ligated at room temperature for 2 hours.
Component
Amount
Ferritin DNA
10 µL
pSB1C3 DNA
10 µL
T4 ligase
0.5 µL
T4 ligase buffer
3 µL
\( \mathrm{H_2O}\)
6.5 µL
Components used for ligation.