Team:Groningen/Notebook/Ligation pg5

Blue Bio Energy
Ligation (pg5)
Ligation was done to combine the PGA DNA and a vector in order to transform it into E. coli.
Make a more negatively charged biofilm.
Ligation was not successful.
Ligation
00:00, 15 July 2015 - 00:00, 15 July 2015
Ligated in an linearized pSB1C3 (digested with EcoRI and PstI)
Marieke Mulder