Team:Groningen/Notebook/Psalt Colony PCR zp20
Blue Bio Energy
Psalt Colony PCR (zp20)
A colony PCR was performed to check the insert of a transformed E. coli.
To create a salt inducible promotor.
Gel results show not the correct insert.
Colony PCR
00:00, 17 July 2015 - 00:00, 17 July 2015
Another 8 colonies were taken from the plate and tested for their insert with a colony PCR. After PCR samples were loaded on 1% agarose gel with SERVA DNA Stain G 1:50000. Gel results don't show the correct insert.