Team:Groningen/Notebook/Psalt Ligation zp12

Blue Bio Energy
Psalt Ligation (zp12)
Ligation was done to combine the salt inducible promotor (proH) into a backbone in order to put it in front of a gene.
To create a salt inducible promotor.
Ligation was not successful.
Ligation
00:00, 10 July 2015 - 00:00, 10 July 2015
Ligation of proH promoter in K823023 and also in pSB1C3. Used 10 ul proH and 5 ul backbone (K823023 and pSB1C3)
Marieke Mulder