Team:Groningen/Notebook/Psalt Ligation zp32

Blue Bio Energy
Psalt Ligation (zp32)
Ligation was done to combine the salt inducible promotor (proH) into a backbone in order to put it in front of a gene.
To create a salt inducible promotor.
Ligation was successful.
Ligation
00:00, 17 August 2015 - 00:00, 17 August 2015
The following vectors and inserts were ligated.
#
Vector used
Insert
1
tasA in pSB1C3
Salt promotor
2
bslA in pSB1C3
Salt promotor
3
pSB1C3
Salt promotor
Ligated constructs
Marieke Mulder