Team:Groningen/Notebook/Psalt Transformation zp23
Blue Bio Energy
Psalt Transformation (zp23)
Transformation was done to introduce the ligated plasmid DNA into E. coli.
To create a salt inducible promotor.
Transformation failed. No colonies on plate.
Heat shock transformation
00:00, 20 July 2015 - 00:00, 21 July 2015
Competent E. coli were transformed with 10 µL ligation product and plated on LB with ampicillin (K823023). Transformation failed. No colonies visible on plate.