Team:Groningen/Notebook/Restriction th3
Blue Bio Energy
Restriction (th3)
Digestion of pdg1664
Extra integration of DNA in thrC locus
Plasmid was digested and purified
Restriction
00:00, 26 Augustus 2015 - 00:00, 26 Augustus 2015
pdg1664 plasmid DNA was digested with EcoRI and AatII for 2 hours at 37 °C. The restriction samples were run on a 1% agarose gel and correct bands were cut out of the gel and purified with the PCR purification kit from Thermo Scientific.