Team:Groningen/Notebook/Restriction with Inserts th21
Blue Bio Energy
Restriction thrC compleet, tasA, bslA and slrR Inserts (th21)
Digestion of thrC completed
Extra integration of DNA in thrC locus
Digested products
Restriction
00:00, 6 September 2015 - 00:00, 6 September 2015
The following components were used.
Construct
Restriction enzymes
ThrC backbone
EcoRI and PstI
Restriction.
The restriction was performed for 2 hours at 37 °C in stopped by heating at 80°C for 20 min.