Team:Groningen/Notebook/tasA Digestion t30
Blue Bio Energy
Digestion tasA, BBa_K823023 and BBa_K823024 (t30)
tasA, BBa_K823023 and BBa_K823024 were digested for ligation.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
tasA, BBa_K823023 and BBa_K823024 were digested.
Restriction
00:00, 9 June 2015 - 00:00, 9 June 2015
For the digestion of tasA and the plasmids (BBa_K823023 and BBa_K823024), a Mastermix was made, which was added to the DNA.
Component
Amount
10x buffer (2.1)
6.6 µL
EcoRI
3.3 µL
PstI
3.3 µL
\( \mathrm{H_2O}\)
52.8 µL
Components Mastermix digestion.
#
Component
Amount
1
Mastermix
15 µL
DNA (tasA DNA)
10 µL
2
Mastermix
15 µL
DNA (K823023)
10 µL
3
Mastermix
15 µL
DNA (K823024)
10 µL
Components digestion samples.
The samples were incubated for 2 hours at 37 °C.