Team:Groningen/Notebook/tasA Gel purification t21
Blue Bio Energy
tasA Ligation product purification (t21)
In this experiment the ligation products of tasA with the BBa_K823023 and BBa_K823024 backbones were purified.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The ligation products were purified.
Gel purification
00:00, 2 June 2015 - 00:00, 2 June 2015
The bands were cut out of the gel and purified with the PCR purification kit.
Sample
Mass gel(mg)
Binding buffer (µL)
tasA
77
144
K823023
181
362
K823024
133
266
Amounts of DNA and binding buffer added.
The samples were eluted with 20 µL elution buffer and stored at -20 °C as:
tasA DNA ligate
K823023 ligate
K823024 ligate