Team:Groningen/Notebook/tasA Ligation t32
Blue Bio Energy
tasA Ligation with BBa_K823023 and BBa_K823024 (t32)
In this experiment tasA was ligated with BBa_K823023 and BBa_K823024.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The ligation step of tasA with BBa_K823023 and BBa_K823024 was performed.
Ligation
00:00, 9 June 2015 - 00:00, 9 June 2015
The ligation of tasA in the plasmids (BBa_K823023 & BBa_K823024) was performed by combining the following components.
Sample
Component
Amount
23 lig
insert (tasA dig)
10 µL
vector(23 dig)
5 µL
10x buffer
2 µL
T4 ligase
0.5 µL
\( \mathrm{H_2O}\)
2.5 µL
24 lig
insert (tasA dig)
10 µL
vector(24 dig)
5 µL
10x buffer
2 µL
T4 ligase
0.5 µL
\( \mathrm{H_2O}\)
2.5 µL
Components of ligation samples.
The mixtures were ligated for 2 hours at room temperature.