Team:Groningen/Notebook/tasA PCR purification t29
Blue Bio Energy
tasA Gel Purification (t29)
In this experiment, the PCR product of tasA was purified for use in ligation.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The PCR product of tasA was purified.
Gel purification
00:00, 8 June 2015 - 00:00, 8 June 2015
The band of tasA was hardly visible, but was nevertheless cut out and purified with the PCR purification kit.
Sample
Mass gel (mg)
Binding buffer (µL)
tasA
270
540
Amounts of DNA and binding buffer added.
The purified DNA was stored as tasA DNA (7.3 ng/µL Nanodrop value).