Team:Groningen/Notebook/tasA PCR purification t8

Blue Bio Energy
tasA PCR purification (t8)
The Gibson assembly products were purified.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The purification of the Gibson assembly products was performed.
PCR purification
00:00, 19 May 2015 - 00:00, 19 May 2015
The Gibson assembly products were purified with the PCR purification kit and stored as tasA DNA 1 and tasA DNA 2 in freezer at -20 °C.
Harm Ruesink