Team:Groningen/Notebook/tasA Primer design t1
Blue Bio Energy
tasA Primer Design t1
Design primers for the isolation of DNA and getting rid of restriction sites within the gene.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
Designing the primers was successful.
None
00:00, 16 April 2015 - 00:00, 16 April 2015
Primers were designed for tasA in B. subtilis 168.
#
Primer
Sequence
5
tasA for
CGGAATTCGCGGCCGCTTCTAGAGCAATAAAAGGGGAGCTTACCATG
6
tasA rev
CGCTGCAGCGGCCGCTACTAGTATTATTAATTTTTATCCTCGCTATGC
7
tasA EcoRI overlap for
GATTTATCTGCTAAAGAGAACTCAGCGAGTGTGAAC
8
tasA EcoRI overlap rev
GTTCACACTCGCTGAGTTCTCTTTAGCAGATAAATC
9
tasA PstI overlap for
GAGTTTAGGAGTTGCTTCAGCAGCACTAGGATTAG
10
tasA PstI overlap rev
CTAATCCTAGTGCTGCTGAAGCAACTCCTAAACTC
Primers for tasA in B. subtilis 168.
Primers for tasA.