Team:Groningen/Notebook/tasA Sequencing t59
Blue Bio Energy
tasA Sequencing (t59)
The tasA DNA was send for sequencing to check if the sequence was correct.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
Sequencing showed that the tasA (T2) sample is correct.
None
00:00, 30 July 2015 - 00:00, 30 July 2015
The transformation of 29 July was checked. No colonies were visible on the LB+cm plate. T2 was send to Macrogen for sequencing.
Component
Amount
1/20 primer (5 pmol/µL)
5 µL
plasmid DNA (T2)
5 µL
Amount of primers and DNA sent for sequencing.
Primer
Code
prefix primer (forward)
17C4ZAD658
suffix primer (reverse)
17C4ZAD659
Sequencing codes Macrogen.
The results from Macrogen showed that T2 has the right sequence.