Team:Groningen/Notebook/tasA Transformation t23
Blue Bio Energy
tasA K823023 Transformation
The tasA K823023 ligation product was transformed into E. coli.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The transformation into E. coli failed.
Heat shock transformation
00:00, 3 June 2015 - 00:00, 3 June 2015
10 µL of the ligation mixture of tasA with K823023 was put in 100 µL competent E. coli (1). Two mixtures were streaked out on an LB+amp plate:
90% plate (90 µL)
10% plate (10 µL)
00:00, 4 June 2015 - 00:00, 4 June 2015
The transformation was checked. No colonies were visible on either plate so the transformation failed.