Team:Groningen/Notebook/tasA Transformation t55
Blue Bio Energy
tasA Heat Shock Transformation (t55)
The heat shock transformation of the ligation product was performed to put the DNA in E. coli.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The ligation did not succeed.
Heat shock transformation
00:00, 23 July 2015 - 00:00, 23 July 2015
The heat shock transformation was performed and the culture was plated on LB+amp plates and incubated overnight at 37 °C.
00:00, 24 July 2015 - 00:00, 24 July 2015
The transformation of 23 July was checked. One white colony was visible on the plate, but this colony did not have the insert. So the ligation failed.