Team:Groningen/Protocols and Protocols/Gel Electrophoresis
Blue Bio Energy
Gel Electrophoresis
Separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.
ML-I
Gel Electrophoresis
1. Prepare 100 ml of agarose gel solution and heat until the solution is completely clear and no small floating particles are visible.
2. Cool the gel to 50-60°C and cast the gel into the gel tray.
3. Add 2 μl of the stain to the gel solution and mix it gently.
4. When the gel is solid, load the samples and perform electrophoresis. Samples should be mixed with 6X loading dye (1 loading dyes: 5 sample).
5. Detect the bands under UV illuminator (detection method: SYBR safe).