Team:Groningen/Protocols and Protocols/restriction
Blue Bio Energy
Restriction
Cutting DNA.
ML-I
Restriction
Component
Plasmid DNA
Unpurified Product
Genomic DNA
Water, nuclease free*
up to 20 μL
up to 20 μL
up to 50 μL
10x NEB 2.1 buffer
2 μL
2 μL**
5 μL
DNA
up to 1 μg
up to 0.2 μg
up to 5 μg
NEB Enzyme
0.5 μL
0.5 μL
0.5 μL
Total Volume
20 μL
20 μL
20 μL
Restriction.