Lexicon
Temperature sensitive cI_857= Mutated variant of the phage lambda repressor protein. It can bind firmly to the lambda promotor and represses transcription at low temperatures, but is unstable at elevated temperatures.
LuxI = enzyme for production an AHL called oxohexanoyl-homoserine lactone. This highly diffusible molecule is used as a communication molecule between cells, and is able to bind with LuxR at the lux promotor to initiate transcription.
LuxR = cytoplasmic receptor for AHL
Ag43 = membrane protein, causes autoaggregation of cells
IlvE = last enzyme in the biosynthesis of leucine. In order for it to work, we need to provide the right substrate:4-methyl-2-oxopentanoate to the medium.
Leucine = amino acid, but also a repellent if tar is knocked out
GFP = green fluorescent protein
YFP = yellow fluorescent protein
RFP = red fluorescent protein
PenI = Repressor protein, binds to the PenI regulated promotor
LVA = degradation tag, signals the linked protein for intracellular degradation
His-tag: repeat of six histidines, in order to purify it with nickelbeads
E-tag: links the tagged protein with an anti-E antibody
