Team:LASATX/Parts




Part Table

PART NAME

PART NUMBER

CO detector GFP reporter system BBa_K1624000
device BBa_K1624001
lacO BBa_K1624002
RBS BBa_K1624003
sfGFP BBa_K1624004
T7 -> CooA BBa_K1624005
T7-lacO-sfGFP BBa_K1624006
Wintergreen Smell BBa_K1624007
pCoof RBS BBa_K1624008


Part Characterization

We have characterized the part BBa_J64997 (T7 promoter sequence, 2007 Voigt Lab).

COWg Fragments

COWg1 T7- CooA- 2½ TT 1,000 bp
COWg2 2½ TT- ⅔ BSMT 1,050 bp
COWg3 ⅓ BSMT-⅓ pchBA 1,050 bp
COWg4 ⅔ pchBA-pCoof 1,222 bp

Plasmids

PCR 2.1 Plasmid T7- CooA- 2½ TT 3,931 bp
pCDF-T7-gfp Plasmid 2½ TT- ⅔ BSMT 4,279 bp
pCDF-pCoof-gfp Plasmid ⅓ BSMT-⅓ pchBA 4,308 bp
pET21 ⅔ pchBA-pCoof 5,731 bp

Other Parts

Promoters

T7 23 bp
pCoof 94 bp
RBS 12 bp

Genes

CooA 669 bp
BSMT 1,074 bp
pchBA 1,736 bp
sfGFP 744 bp

Other

TT 129 bp

New Parts

pCooF-RBS

Short Description: promoter and rbs, activated by CooA transcription factor

Long Description: part of the pCooF operon and ribosome binding site downstream, activated by the CooA transcription factor bound to carbon monoxide.

T7-Wintergreen

Short Description: continual expression of a wintergreen fragrance

Long Description: the wintergreen pathway under constituent promoter T7. pchBA and BMST genes convert chorismate to salicylic acid the methyl salicylate, the desired wintergreen fragrance.

Whole Construct

Short Description: responds to presence of carbon monoxide with wintergreen smell

Long Description: CooA transcription factor binds carbon monoxide, and this CooA-CO complex activates the pCooF promoter, which regulates expression of the wintergreen pathway. The wintergreen pathway consists of the pchBA and BSMT genes. This pathway converts chorismate, an endogenous molecule in E. coli bacteria to salicylic acid with the pchBA protein, and salicylic acid to methyl salicylate, or the wintergreen fragrance, with the BSMT protein. Thus in the presence of carbon monoxide, this part creates a wintergreen fragrance.