Team:London Biohackspace/protocols/yepd-agar-plates


Preparing YEPD agar plates

1. For 1 litre of YEPD media measure the following into an erlenmayer or duran flask

Bacto yeast extract (Difco 0127-17) (1%) 10g
Bacto peptone (Difco 0118-17) (2%) 20g
Glucose (2%) 20g

Available premixed as Difco 0428-17.

Bacto agar (Difco 0140-01) (2%) 20g

2. Autoclave for at least 15 minutes at 121C
3. Pour 20ml into an agar plate. Leave to cool at room temperature for 2-3 hours and store at -4C