Team:Marburg/Protocols/Protocol23

Yeast Miniprep

  • pellet 10ml of ON yeast culture
  • resuspend in 600 μl water
  • add 100 μl lysis buffer
  • add 1Volume beads
  • 2 min bead beating - 8000rpm
  • use hot needle to make hole into tube to get rid of beads
  • centrifuge (5min, 4000 rpm)
  • add 350 μl neutralisation buffer
  • centrifuge (5min, 4000 rpm)
  • apply supernatant to column
  • centrifuge (5min, 4000 rpm)
  • apply 200 μl Endo wash buffer
  • centrifuge (5min, 4000 rpm)
  • apply 400 μl wash buffer
  • centrifuge (5min, 4000 rpm)
  • dry on heating block for 10min
  • heat water
  • add 20 μl hot water
  • centrifuge (2min, 13000rpm)
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