Team:Marburg/Protocols/Protocol29

Ligation

Prepare the following reaction mixture (total volume: 20 μL):

Ligation Mix
100 ng Vector DNA
300 ng Insert DNA
2 μL T4 DNA Ligase Buffer
1 μL T4 DNA Ligase
X μL (fill up to 20 μL) dd H2O

Incubate for 30 min at 37°C


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