Team:NAIT Edmonton/Logbook

Team NAIT 2015

Log Book

First Day in Lab!

Our team got the overview of our work space for the summer. We were geared and ready to start the iGEM adventure!

May 4

Research into Silver Staining

Before beginning a project, it is of great importance to fully understand what it is you are planning to research. Our team focused on understanding the basics and chemistry of silver staining completely.

May 4 to 8

Formation of Color in Silver Staining

What was the scientific basis of color formation post silver staining?

May 11 to 15

Spruce Grove High School Lab

Students taking Biology 30 at SGHS came to NAIT today and we were lucky enough to be able to T.A for them.

May 21

Alberta Innovates and geekStarter

Team NAIT went on a road trip to the University of Calgary!

May 24

Research Continued...

Getting our wiki set up and looking for sponsorships.

May 25 to 29

Getting Our Timeline Set Up

Time is ticking! We are already into the second month of the summer!

June 1 to 5

techLife and NAIT Photoshoot

We had a photoshoot with NAIT's techLife magazine. Thank you to Ms. Linda Hoang and Blaise for your patience!

June 11

Ongoing Research and Practice

Perfecting our silver stain techniques!

June 12

Sequences Ordered!

First four sequences of our own design ordered from IDT!

June 16

Met the President!

No, not Obama. We met NAIT's President! Dr. Glenn Felthan and NAIT's VP External and CDO, Mr. George Andrews were most accommodating.

June 18

Article on Synthetic Biology in Canada

Submitted an article for Team iGEM Amoy China.

June 20

Plasmid and Sequence Digestion

A plasmid double digestion was performed along with digestion of four of our sequences. The sequences used were Arginine, Lysine, Glutamic Acid and Cysteine samples.

June 22

Sequences Arrived!

June 24

Research, Presentations and Practice

"If we knew what it was we were doing, it would not be called research, would it?" - Albert Einstein

June 22 to 25

Presentation to the Office of Research and Innovation

Today, we were lucky enough to have ORnI hear, assess and critique our preliminary presentation for iGEM. We are so happy to know that we have the full support of so many amazing individuals at the Office of Research and Innovation and at NAIT.

June 26

Happy Canada Day!

July 1

Pumping out those Sequences

This week, our team really focused on creating a variety of sequences for testing. Equipped with the knowledge from our literature searches in prior weeks, we crafted each sequence carefully and diligently. As a team, we created and ordered over 25 this week!

June 29 to July 3

And so the cloning begins...

Dr. Marcet helped us get started with cloning! But first, we had to test our enzymes to see if they will properly linearize our plasmid!

July 6

Expressing our Custom Sequences in our E. coli

Got our sequences inserted (hopefully) and now we want to grow our bacteria!

July 10

Team BBQ!

We had an awesome Sunday barbecue! The sun was shining bright, we had cold drinks and great company - A perfect day.

July 12

Maya Monday

Slowly cranking out those animations! Stay tuned!

July 13

Experiment and Protocols Flowchart

Drafted and published the first version of our experimental protocols and design! You can view it here.

July 16

Officially Registered!

SO EXCITED!!!!!!!!!

July 20

AITF and geekStarter Workshop

NAIT is off for the 6 hour drive to visit a workshop hosted by our co-Albertan team, Team Lethbridge!

July 24 to 26

Team Re-Branding

Today, we stepped away from our original iGEM inspired logo and tried to come up with a new team identity so that we could stand out from the crowd and our team would leave a lasting impression in iGEM.

July 30

NAIT Photoshoot and Mini-Prep

We had another photo shoot with our institution! Eduardo and Joy managed to escape early, because of a plasmid mini-prep.

July 31

Let's Work Together!

Our team is new to iGEM so we are helping out others as best we can. We've completed many surveys and given feedback to many teams!

August 6

Title and Abstract.. Submitted!

Today was the due date for the final abstract and title for iGEM's brochures and websites.

August 7

Officially Argentum!

Received our new logo concepts from our friend and design advisor, Derek Lue. We changed the layout of our Wiki to what you see now!

August 10

Abbie's Back!

Abbie’s last day working her full-time summer student position as a lab tech for Keyera AEF. Time to get into the lab and work on the project!

August 14

Go, Team NAIT, GO!

We're getting an overwhelming amount of support from NAIT. We're even getting golf shirts to wear in Boston!

August 17

Argentum ... Re- IM[Ag]INE 'd

Turns out, we cannot use Argentum as our team name. There is a local company that has trademarked that brand and we do not want to get into any trouble.

August 19

Watch our Intro Video!

We have a new respect for anyone who has to be in front of the camera as their career.. This less-than-two-minutes video took us a few hours to shoot and edit.

Watch it! August 20

iGEM Banner

Final banner design finished and ready for sending to iGEM HQ. We cannot wait to see our banner hanging at the Hynes Convention Centre.

August 21

Collaboration with our New Application Track

As you know, we're a first-time team. We reached out to our Track mates and asked them to help us out.

August 26-28

Digestion of Sequences

All our sequences (23) were digested and in doing so each were cleaved at specific sites to later ligate with our plasmid, pSB1C3.

August 26

IDT Interview

We got interviewed by one of our primary sponsors, IDT!

Read more August 27

Digestion of Plasmids + rSAP

We digested our pSB1C3 plasmids to cleave each at specific restriction sites to be able to insert our sequences of interest.

August 28

Presentation.. Round Two!

We presented to NAIT's Office of Research and Innovation again to give them a rundown on our current progress for our project.

August 28

Sequence and Plasmid Ligation

Ligation was conducted on our sequences of interest to join and bind with our plasmid pSB1C3 (T7+RBS) backbone.

August 29


From ligation, all novel sequences were transformed and plated. We counted a total of 302 colonies from this experiment. (THEY ARE ALIVE!!!!)

August 31 to September 2

Colony PCR

Four 96 well plates and many agarose gels later... We obtained the colonies that showed to have taken up our sequences!

September 2

Grew Our Colonies

To ensure our colonies would produce adequate protein when induced, we prepared them for harvest during their log phase of growth.

September 5

IPTG Induction of Proteins

We're starting to see the light at the end of the tunnel! We induced our proteins with IPTG when they had an absorbance of approximately 0.8.

September 8

Back to School for NAIT students!


september 8

Ni NTA Kit Purification!

Received our Ni-NTA kit from the University of Alberta.

September 10

IM[Ag]INE Shirts have Arrived!

Additionally, we finished pelleting and purifying more samples of our proteins. Unfortunately, not all of them showed a concentration in the NanoDrop.

September 11

Preliminary Results

After four months of work, we completed the last step of our experimental design - SDS PAGE and Silver Staining of our synthetic proteins.

September 13

Experimental Validation

We ran more gels in order to validate our preliminary results. Regrettably, the gels failed to run properly and we were unable to finish in time.

September 14

The End of our Lab Journey

And so it ends. Today, we pulled the plug on all remaining lab work and running experiment. Now, it's time to focus on our Wiki, Poster and Presentation.

September 15

Wiki FREEZE!!!!!

Had a blast browsing through our fellow iGEM team's tweets about #WikiFreeze. We're definitely scrambling. AHHH!!!!

September 18

DestiNAITion: Boston

September 18