Team:NRP-UEA-Norwich/Parts

House of Carbs

Parts

BASIC PARTS FOR ENGINEERING BACTERIAL GLYCOGEN

Part Contents Backbone Description Function Name in registry
GlgXpSB1C3Debranching Enzyme (E. coli)Coding sequence only BBa_K1618025
GlgBpSB1C3Branching Enzyme (E. coli)Coding sequence only BBa_K1618000
MAOpSB1C3Acyltransferase Candidate 1 Coding sequence only BBa_K1618001
Rv3030pSB1C3Acyltransferase Candidate 2Coding sequence onlyBBa_K1618002
Rv3034cpSB1C3Acyltransferase Candidate 3Coding sequence only BBa_K1618003
Rv3037cpSB1C3Acyltransferase Candidate 4Coding sequence only BBa_K1618004

COMPOUND PARTS FOR ENGINEERING BACTERIAL GLYCOGEN

Part Contents Backbone Description Function Name in registry
P-Lac(R0010)_RBS(B0034)_GlgX(K1618025)pSB1C3Debranching Enzyme Expression ConstructRemoves Glycogen BranchesBBa_K1618026
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)pSB1C3Branching Enzyme Expression ConstructAdds Glycogen BranchesBBa_K1618022
P-Lac(R0010)_RBS(B0034)_MAO(K1618001)pSB1C3Acyltransferase Expression ConstructTransfers Acyl Groups to GlycogenBBa_K1618005
P-Lac(R0010)_RBS(B0034)_Rv3030(K1618002)pSB1C3Acyltransferase Expression ConstructTransfers Acyl Groups to GlycogenBBa_K1618007
P-Lac(R0010)_RBS(B0034)_Rv3034c(K1618003)pSB1C3Acyltransferase Expression ConstructTransfers Acyl Groups to Glycogen BBa_K1618008
P-Lac(R0010)_RBS(B0034)_Rv3037c(K1618004)pSB1C3Acyltransferase Expression ConstructTransfers Acyl Groups to Glycogen BBa_K1618009
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3030(K1618002)pSB1C3Branching Enzyme + Acyltransferase Expression ConstructTransfers Acyl Groups to GlycogenBBa_K1618023
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3034c(K1618003)pSB1C3Branching Enzyme + Acyltransferase Expression ConstructTransfers Acyl Groups to GlycogenBBa_K1618024
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3037c(K1618004)pSB1C3Branching Enzyme + Acyltransferase Expression ConstructTransfers Acyl Groups to GlycogenBBa_K1618027

COMPOUND PARTS FROM THE BUTYRATE BIOSYNTHESIS PATHWAY (Coding sequences from Coprococcus sp. L2-50 -DSM 16842-)

Part Contents Backbone Description Function Name in registry
P-Lac(R0010)_RBS(B0034)_BukpSB1C3Butyrate Kinase Expression Contruct Catalyzes the Conversion of Butyrate to/from ButyrylBBa_K1618012
P-Lac(R0010)_RBS(B0034)_PTBpSB1C3Phosphotransbutrylase Expression ConstructCatalyzes the Conversion of Butyryl to/from Butyryl-CoABBa_K1618013
P-Lac(R0010)_RBS(B0034)_Buk_PTBpSB1C3Butyrate to Butyryl-CoA Pathway Catalyzes the Conversion of Butyryl to/from Butyryl-CoABBa_K1618020
P-Lac(R0010)_RBS(B0034)_THLpSB1C3Thiolase Expression ConstructCatalyzes the Conversion of Acetyl-CoA to Acetoacetyl-CoA BBa_K1618014
P-Lac(R0010)_RBS(B0034)_CROpSB1C3Crotonase Expression ConstructCatalyzes the Conversion of 3-hydroxybutyryl-CoA to Crotonyl-CoA BBa_K1618015
P-Lac(R0010)_RBS(B0034)_BHBDpSB1C3β-hydroxybutyryl-CoA Dehydrogenase ExpressionCatalyzes the Conversion of Acetoacetyl-CoA to 3-hydroxybutyryl-CoA BBa_K1618016
P-Lac(R0010)_RBS(B0034)_BCDpSB1C3Butyryl-CoA Dehydrogenase Expression Construct Catalyzes the Conversion of Crotonyl-CoA to Butyryl-CoA BBa_K1618017
P-Lac(R0010)_RBS(B0034)_EFTbpSB1C3Electron Transfer Protein β Expression ConstructCatalyzes Conversion of Acetate to ButyrateBBa_K1618018
P-Lac(R0010)_RBS(B0034)_EFTapSB1C3Electron Transfer Protein α Expression ConstructCatalyzes Conversion of Acetate to ButyrateBBa_K1618019
P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD-BCD-EFTb-EFTapSB1C3Acetyl-CoA to Butryl-CoA PathwayCatalyzes Conversion of Acetyl-CoA to Butryl-CoA BBa_K1618021
P-Lac(R0010)_RBS(B0034)_Buk-PTB-THL-CRO-BHBD-BCD-EFTb-EFTapSB1C3Acetyl-CoA to Butyrate Complete Biosynthetic PathwayCatalyzes Conversion of Acetyl-CoA to ButyrateBBa_K1618046

BASIC AND COMPOUND PARTS FOR ENGINEERING PLANT STARCH

Part Contents Backbone Description Function Name in registry
P-35s(K1467101)_TP(K1618037):MAO(K1618001)_T-35s(BBa_K1618037)pSB1C3*Acyltransferase Candidate 1 Expression ConstructTransfers Acyl Groups to StarchBBa_K1618033
P-35s(K1467101)_TP(K1618037):Rv3030(K1618002)_T-35s(BBa_K1618037)pSB1C3*Acyltransferase Candidate 2 Expression ConstructTransfers Acyl Groups to StarchBBa_K1618034
P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003)_T-35s(BBa_K1618037)pSB1C3*Acyltransferase Candidate 3 Expression ConstructTransfers Acyl Groups to StarchBBa_K1618035
P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004)_T-35s(BBa_K1618037)pSB1C3*Acyltransferase Candidate 4 Expression ConstructTransfers Acyl Groups to StarchBBa_K1618036
P-35s(K1467101)_TP(K1618037):MAO(K1618001):GFP(K1467204)_T-35s(BBa_K1618037)pSB1C3*Acyltransferase Candidate 1 -Fused to GFP -Expression Construct Check Localization in Plastid of Candidate GeneBBa_K1618029
P-35s(K1467101)_TP(K1618037):Rv3030(K1618002):GFP(K1467204_T-35s(BBa_K1618037)pSB1C3*Acyltransferase Candidate 2 -Fused to GFP -Expression Construct Check Localization in Plastid of Candidate GeneBBa_K1618030
P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003):GFP(K1467204_T-35s(BBa_K1618037)pSB1C3*Acyltransferase Candidate 3 -Fused to GFP -Expression Construct Check Localization in Plastid of Candidate GeneBBa_K1618031
P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004):GFP(K1467204_T-35s(BBa_K1618037)pSB1C3*Acyltransferase Candidate 4 -Fused to GFP -Expression Construct Check Localization in Plastid of Candidate GeneBBa_K1618032
35s TerminatorpSB1C3**Eukaryotic Terminator from the 35s Gene (Cauliflower Mosaic Virus)Terminates Transcription in Plant CellsBBa_K1618037
Chloroplast Transit PeptidepSB1C3***Synthetic Plastid Transit Peptide From the Consensus of Multiple RuBisCo Small Subunit GenesIn an N-terminal Fusion, this Peptide Directs Proteins to the Plant Plastid.BBa_K1618028

* This was converted to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467400 (complete transcriptional unit flipper) made by the NRP-UEA 2014 team. These parts were tested by cloning into a binary plasmid vector backbone prior to delivery to plants leaves using Agrobacterium tumefaciens mediated transfection.

** This was converted to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467300 (Terminator Flipper) made by the NRP-UEA 2014 team.

*** This is assembled in a Golden Gate Universal Acceptor Plasmid (pUAP1) in the pSB1C3 backbone, according to RFC 106. It is a Golden Gate part in the plant standard syntax, not a BioBrick.

MANY THANKS TO OUR SPONSORS

Useful Links

Contact Us

  • nrpuea.igem2015@gmail.com

  • Norwich Research Park,
  • Colney,
  • Norwich, NR4 7UH, UK.

We are the NRP-UEA-Norwich 2015 iGEM Team.

Designed and developed by the NRP-UEA-Norwich iGEM Team