Team:Paris Bettencourt/Protocols/Yeast lysis with NaOH
Ferment It Yourself
iGEM Paris-Bettencourt 2O15
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Yeast lysis with NaOH
20 µl NaOH into PCR tubes
Pick colonies into NaOH
Incubate at 95°C for ~45 minutes
Centrifugate at 8000 krpm for 10 minutes
Use 1 µl supernatant as template in a (10 µl) PCR