Team:Paris Saclay/Notebook/August/27
Tuesday 27th August
Lab Work
Electrophoresis
by Audrey
Verification of PCR products:
- BBa_K1707013
- BBa_K1707019
- BBa_K1707020
- BBa_K1707030
- BBa_K1707035
- BBa_K1707036
- BBa_K1707021
- BBa_K1707027
- Controls (-)
Agarose gel 1%, Migration 115V
Verification of PCR products, from left to right: 1. DNA Ladder, 2. BBa_K1707036, 3. BBa_K1707021+DMSO, 4. BBa_K1707021, 5. BBa_K1707027+DMSO, 6. BBa_K1707027, 7. Reverse oligo control BBa_K1707013+DMSO, 8. Reverse oligo control BBa_K1707013, 9. Forward oligo control BBa_K1707013+DMSO, 10. Forward oligo control BBa_K1707013
Verification of PCR products, from left to right: 1. DNA Ladder, 2. BBa_K1707013+DMSO, 3. BBa_K1707013, 4. BBa_K1707019+DMSO, 5. BBa_K1707019, 6. BBa_K1707020+DMSO, 7. BBa_K1707020, 8. BBa_K1707030+DMSO, 9. BBa_K1707030, 10. BBa_K1707035+DMSO, 11. BBa_K1707035, 12. BBa_K1707036+DMSO
We can conclude that:- BBa_K1707035 and BBa_K1707036 aren't OK, we will have to start again
All of the next one (+DMSO) are OK:
- BBa_K1707013
- BBa_K1707019
- BBa_K1707020
- BBa_K1707030
- BBa_K1707021
- BBa_K1707027
Digestion
by Audrey
PCR products of: (+DMSO)
- BBa_K1707013
- BBa_K1707019
- BBa_K1707020
- BBa_K1707030
- BBa_K1707021
- BBa_K1707027
- BBa_K1707036
Mix in each tube:
- 1 μL DpnI
- 45μL PCR Product
- 5,5 μL FastDigest Buffer
- 3,5 μL H2O
Incubation 1h30, 37°C
Digestion
by Audrey
- BBa_K1707022
Mix:
- 1 μL XbaI
- 1 μL PstI
- 15 μL plasmid
- 2 μL FastDigest Buffer
- 1 μL H2O
- BBa_K1707023
Mix:
- 1 μL SpeI
- 1 μL PstI
- 15 μL Plasmid
- 2 μL FastDigest Buffer
- 1 μL H2O
Incubation 1h30, 37°C
Member present:
- Instructors: Claire
- Students: Audrey
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
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