Team:Paris Saclay/Notebook/August/28

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Friday 28th August

Lab Work

Well 2: Verification by digestion with XbaI and EcoRI, Wells 3-12: Quantification of reverse PCR products; from left to right: 1. DNA Ladder, 2. BBa_K1707037#9, 3. BBa_K1707013+DMSO, 4. BBa_K1707019+DMSO, 5. BBa_K1707020+DMSO, 6. BBa_K1707030+DMSO, 7. BBa_K1707036+DMSO, 8. BBa_K1707021+DMSO, 9. BBa_K1707021, 10. BBa_K1707027+DMSO, 11. BBa_K115017 (10%), 12. BBa_K115017 (100%)

Wells 2-6: Verification by digestion of BBa_K1707037, Wells 7-12: Verification of PCR products; from left to right: 1. DNA Ladder, 2. BBa_K1707037#5, 3. BBa_K1707037#6, 4. BBa_K1707037#7, 5. BBa_K1707037#8, 6. BBa_K1707037#9, 7. BBa_K1707035+DMSO, 8. BBa_K1707035+DMSO, 9. BBa_K1707035+DMSO, 10. BBa_K1707036+DMSO, 11. BBa_K1707036+DMSO, 12. BBa_K1707036+DMSO

Wells 2-6: Verification by digestion of BBa_K1707037, Wells 7-12: Verification of PCR products; from left to right: 1. DNA Ladder, 2. BBa_K1707037#5, 3. BBa_K1707037#6, 4. BBa_K1707037#7, 5. BBa_K1707037#8, 6. BBa_K1707037#9, 7. BBa_K1707035+DMSO, 8. BBa_K1707035+DMSO, 9. BBa_K1707035+DMSO, 10. BBa_K1707036+DMSO, 11. BBa_K1707036+DMSO, 12. BBa_K1707036+DMSO

Member present:

• Instructors: Claire
• Students: Audrey

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t@iGEMParisSaclay
aUniversité Paris Sud
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*This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.