Team:Paris Saclay/Notebook/July/27
Contents
Monday 27th July
Lab Work
New culture
by Seong Koo
Biobricks:
- BBa_K1707005
- BBa_K1707006
- BBa_K1707007
Ligation
by Pauline and Coralie
- BBa_K1707008
- 2µL Tp 10x
- 1µL Ligase
- 11µL BBa_K1707003
- 6µL BBa_J23101
- BBa_K1707010
- 1µL Tp 10x
- 1µL Ligase
- 12µL BBa_K1399005
- 10µL BBa_B0015
- 1µL H20
- BBa_K1707009
- 2µL Tp 10x
- 2µL Ligase
- 10µL BBa_S03518
- 6µL BBa_R0051
- BBa_K1707004
- 3µL Tp 10x
- 2µL Ligase
- 8µL BBa_K1707003
- 7µL BBa_R0051
Transformation
by Pauline and Coralie
Biobricks:
- BBa_K1707008
- BBa_K1707010
- BBa_K1707009
- BBa_K1707004
Digestion + Purification + Quantification
by Audrey
Biobricks:
- BBa_R0051
- BBa_K1707000
Digestion: as usual, with SpeI + PstI
Migration: Agarose gel 1%, migration 100V
Plasmid extraction
by Pauline
Biobricks:
- BBa_K1707003 #4 et #5
With Plasmid DNA purification kit from Macherey-Nagel
Digestion + Purification on agarose gel
by Pauline
Biobricks:
- BBa_K1707003 #4 and #5
Mix:
- 10µL plasmid BBa_K1707003
- 1µL XbaI
- 1µL PstI
- 2µL Buffer FastDigest
- 6µL H2O
Purification on agarose gel 1%, migration 100V
Wells 2-3: Quantification, Wells 9-10: Verification of purification; from left to right: 1. DNA Ladder, 2. BBa_K1707000#2, 3. BBa_50051, 4. Empty, 5. Empty, 6. Empty, 7. Empty, 8. Empty, 9. BBa_K1707003#1, 10. BBa_K1707003#2, 11. Empty 12. Empty
Cut with a scalpel Purification with the PCR Clean up kit from Macherey-NagelNew Culture
by Johan
Strains:
- 1696
- 1693
- 1320
in 150mL LB without any antibiotic
Member present:
- Instructors: Alice
- Students: Coralie, Audrey, Pauline, Johan and Seong-Koo
Contact
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. All rights reserved All inspirations encouraged.
*This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. *This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.