To indirect prove wound healing, we perform experiments in cell migration. Two cells are selected for the experiment: Squamous keratinocyte called HaCat. To make the result closer to human, both cells were sampled from human cells.
Squamous keratinocyt cell shows the influence of our peptides on epithelial. On other hand, because angiogenesis is one of the indicators of wound healing, Dermal microvascular endothelium cell is selected here.
In cell migration experiment, samples are divided into following groups:1. Mock2. pQE-60 has no insert and no IPTG induction of LB medium for 200 l (Control 1)3. pQE-60 has insert, without IPTG induction of LB medium for 200 l (Control 2)4. pQE-60 has no insert, with IPTG induction of LB medium for 200 l (Control 3)5. 1l AMPs 6. 5l AMPs7. 100l AMPs8. 200l AMPsFurthermore, all of above groups are divided into three more groups: Epinecidin-1, Signiferin, Epinecidin-1 and Signiferin.