Team:UCLA/Notebook/Honeybee Silk/4 May 2015

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5/4 Transformation of Silk and Prom + Silk

• Using Sri’s chemically competent cells
• Using ligation product from 4/30 (not purifying the ligation product as per Eric’s recommendation)

1. Pre heat plates and SOC at 37 C
2. Thaw 2 aliquots of 50 uL of competent cells on ice for 10 minutes
3. Add 2 ul of non-purified ligated product
4. Incubate for 10 minutes on ice
5. Rescue in 200 uL SOC at 37°C for ~1 hour
6. Plate 100 uL in 1:1 and 1:10 ratios onto chloramphenicol plates
7. Spread with beads
8. Incubate plates at 37 C (put in at 3:30)