Team:UCLA/Notebook/Spider Silk Genetics/ICA Oligo Sequences
ICA Primers
Sequence | Length (bp) | |
---|---|---|
Post-Elution Primer (Forward) | 5'-GAA TTC GCG GCC GCT TC-3' | 17 |
Post-Elution Primer (Reverse) | 5'-CAG TCT GCA GCG GCC G-3' | 16 |
MaSp2 Seq Primer F | 5'-TAC GGT CCT GGT CAA CAG-3' | 18 |
MaSp2 Seq Primer R | 5'-CTG TTG ACC AGG ACC GTA-3' | 18 |
MaSp1 Seq Primer F | 5'-CTA GGA TCT CAG GGA GCA-3' | 18 |
MaSp1 Seq Primer R | 5'-TGC TCC CTG AGA TCC TAG-3' | 18 |
ICA Oligos Sequences (Val sticky ends)
Sequence | Length | |
---|---|---|
Initiator-F | /52-Bio/actggaattcgcggccgcttctagATGcatcatcaccatcaccaccatcataagcttgc | 59 |
Initiator-A-R | /5Phos/AACTgcaagcttatgatggtggtgatggtgatgatgCATctagaagcggccgcgaattc | 59 |
Terminator-A-F | /5Phos/AGTTggatcctaataaTACTAGTAGCGGCCGCTGCAGACTG | 41 |
Terminator-R | CAGTCTGCAGCGGCCGCTACTAGTAttattaggatcc | 37 |
A-cap | /5Phos/agttGACCCGTCACGCTCGAGTATCGTAACTCGAGCGTGACGGGTC | 46 |
B-cap | /5Phos/tgtcGACCCGTCACGCTCGAGTATCGTAACTCGAGCGTGACGGGTC | 46 |
C-cap | /5Phos/cgtgGACCCGTCACGCTCGAGTATCGTAACTCGAGCGTGACGGGTC | 46 |