Team:UCLA/Notebook/Spider Silk Genetics/ICA Oligo Sequences

iGEM UCLA




ICA Primers

Sequence Length (bp)
Post-Elution Primer (Forward) 5'-GAA TTC GCG GCC GCT TC-3' 17
Post-Elution Primer (Reverse) 5'-CAG TCT GCA GCG GCC G-3' 16
MaSp2 Seq Primer F 5'-TAC GGT CCT GGT CAA CAG-3' 18
MaSp2 Seq Primer R 5'-CTG TTG ACC AGG ACC GTA-3' 18
MaSp1 Seq Primer F 5'-CTA GGA TCT CAG GGA GCA-3' 18
MaSp1 Seq Primer R 5'-TGC TCC CTG AGA TCC TAG-3' 18

ICA Oligos Sequences (Val sticky ends)

Sequence Length
Initiator-F /52-Bio/actggaattcgcggccgcttctagATGcatcatcaccatcaccaccatcataagcttgc 59
Initiator-A-R /5Phos/AACTgcaagcttatgatggtggtgatggtgatgatgCATctagaagcggccgcgaattc 59
Terminator-A-F /5Phos/AGTTggatcctaataaTACTAGTAGCGGCCGCTGCAGACTG 41
Terminator-R CAGTCTGCAGCGGCCGCTACTAGTAttattaggatcc 37
A-cap /5Phos/agttGACCCGTCACGCTCGAGTATCGTAACTCGAGCGTGACGGGTC 46
B-cap /5Phos/tgtcGACCCGTCACGCTCGAGTATCGTAACTCGAGCGTGACGGGTC 46
C-cap /5Phos/cgtgGACCCGTCACGCTCGAGTATCGTAACTCGAGCGTGACGGGTC 46