Team:UCLA/Parts

iGEM UCLA




<groupparts>iGEM015 UCLA</groupparts>

PARTS

Honeybee Parts

  1. BBa_K1763000 This part is the coding region of honeybee silk protein #3. For the sequence click here . Click on the link for more details on how the sequence was designed and chosen, as well as how the cloning was carried out.
  2. BBa_K1763001 This part is a composite part of the honeybee silk protein #3 coding region along with BBa_R0010 Promoter and RBS BBa_B0034 . For the sequence click here . Click HERE for more details on how the sequence was designed and chosen, as well as how the cloning was carried out.
  3. BBa_K1763008. This sequence is a fusion protein between our honeybee silk and the Spycatcher, protein which has specific affinity for SpyCatcher. It also contains regulatory elements like a promoter and rbs for expression.
  4. BBa_K1763007 is our honeybee silk sequence with a T7 promoter upstream for effective protein expression in bacterial strains containing t7 polymerase.
  5. BBa_K1763015 is our honeybee silk sequence+sfGFP with a T7 promoter and RBS upstream that can be used in bacterial strains that contain T7 polymerase.

Spider Silk Parts

Basic ICA Parts: Monomers

  1. BBa_K1763002: Major Ampullate Spidroin 2, construct AB. Used for Iterative Capped Assembly of Spider Silk Genes.
  2. BBa_K1763003: Major Ampullate Spidroin 2, construct BC. Used for Iterative Capped Assembly of Spider Silk Genes.
  3. BBa_K1763004: Major Ampullate Spidroin 2, construct CA. Used for Iterative Capped Assembly of Spider Silk Genes.
  4. BBa_K1763009: Major Ampullate Spidroin 2 Sequencing Core, construct AB. Used in Iterative Capped Assembly of Spider Silk Genes as a unique sequencing primer binding site.
  1. BBa_K1763010: Major Ampullate Spidroin 1, construct AB. Used for Iterative Capped Assembly of Spider Silk Genes.
  2. BBa_K1763011: Major Ampullate Spidroin 1, construct AB. Used for Iterative Capped Assembly of Spider Silk Genes.
  3. BBa_K1763012: Major Ampullate Spidroin 1, construct AB. Used for Iterative Capped Assembly of Spider Silk Genes.
  4. BBa_K1763423: Major Ampullate Spidroin 1 Sequencing Core, construct AB. Used in Iterative Capped Assembly of Spider Silk Genes as a unique sequencing primer binding site.

Spider Silk MaSp ICA Constructs

The following constructs were created using Iterative Capped Assembly (ICA) to assemble MaSp monomers.

  1. BBa_K1763424: MaSp2-3(1C3)
  2. BBa_K1763425: MaSp2-3(T7)
  3. BBa_K1763426: MaSp2-6(1C3)
  4. BBa_K1763427: MaSp2-6(T7)
  5. BBa_K1763428: MaSp2-9(1C3)
  6. BBa_K1763429: MaSp2-9(T7)
  7. BBa_K1763430: MaSp2-12(1C3)
  8. BBa_K1763431: MaSp2-12(T7)
  9. BBa_K1763432: MaSp2-15(1C3)
  10. BBa_K1763433: MaSp2-15(T7)
  11. BBa_K1763434: MaSp1-9(1C3)
  12. BBa_K1763435: MaSp1-9(T7)
  13. BBa_K1763436: MaSp1-12(1C3)
  14. BBa_K1763437: MaSp1-12(T7)
  15. BBa_K1763438: MaSp1/2[2:1]-12(1C3)
  16. BBa_K1763439: MaSp1/2[2:1]-12(T7)
  17. BBa_K1763440: MaSp1/2[1:1]-12(1C3)
  18. BBa_K1763441: MaSp1/2[1:1]-12(T7)
  19. BBa_K1763442: MaSp1/2[1:2]-12(1C3)
  20. BBa_K1763443: MaSp1/2[1:2]-12(T7)

Protein Cage Parts

  1. BBa_K1763421 Protein Cage Mutant 10

Silk Functionalization Parts

  1. BBa_K1763444: Silk Co-spinning Module