Team:York/Protocols/Growth Media
Lysogeny Broth
Materials
- 10g of tryptone
- 5g of yeast extract
- 10g of NaCl
- 1L of Deionized Water
- 1M NaCl
- 1M KOH
Procedure
- Use a container with at least double the volume of the liquid that you are making.
- Dissolve 10 g tryptone, 5 g yeast extract, and 10 g NaCl in 950 mL deionized water.
- Adjust the pH of the medium to 7.0 using 1M NaOH or KOH and bring volume up to 1 liter.
- Autoclave.
- Store at room temperature or +4°C.
LB Agar
Procedure
- follow steps to make lysogeny broth as above
- add 15g of agar powder per litre of LB
- autoclave in 200 or 250 mL aliquots in 500mL duran bottles
- add antibiotic desired to melted agar (~55°C)
- pour into petri dishes and leave to harden in asceptic fume hood
- invert, label, wrap with parafilm and store at 4°C