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Buffer

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Name Detail

Phusion HF Buffer NEB

Phusion GC Buffer NEB

10x BSA Buffer 440 mM Tris HCl 8.1; 40 µg/ µL BSA; 2% v/v Glycerol; 300 mM KCl; 200 mM MgCl2

2x Loading Dye 95 % v/v Formamid; 5 % 10 x TBE Buffer (Roth); 1 Tip of a spatula of Bromphenol Blue and Xylenxylanol

10x Transcription Buffer 100 mM Spermidine 400 mM Tris HCl pH 8.1; 100 mM Spermidine; 220 mM MgCl2; 0.1 % Triton X

10x Transcription Buffer 10 mM Spermidine 400 mM Tris HCl pH 8.1; 10 mM Spermidine; 220 mM MgCl2; 0.1 % Triton X

10x Transcription Buffer w/o Spermidine 400 mM Tris HCl pH 8.1; 220 mM MgCl2; 0.1 % Triton X

10x Tris HCl/ KCl Buffer for in vitro transcription 440 mM Tris HCl pH 8.1; 300 mM KCl; 200 mM MgCl2

10x TBE Tris HCl; EDTA; Boric Acid

2x Renaturing Buffer 40 mM HEPES, pH 7.5; 125 mM KCl

DFHBI Solution 198 µl DMSO; 1 mg DFHBI