Template:IONIS Paris/Notebook/03-09-15
Miniprep on liquid culture
Cf liquid culture of the 02/09/15
Digestion of pDawn I-V purified
| Tube | pDawn i-V |
|---|---|
| Buffer 2.1 | 1,2 µL |
| DNA | 10 µL |
| EcoRI | 0,5 µL |
| PstI | 0,5 µL |
37°C, 1h - heat kill: 80°C, 20min
Liquid culture on plates 96 wells of transformed bacteria with pDawn-GFP
1 colonie / well with 100 µl of LB, 37°C, 120 rpm and light exposition
DO405 and DO595 to determine the presence of GFP Liquid culture into 100 ml Erlenmeyer of the 4 most interesting colonies.
3 September 15